Bio-rad Driver



Mouse anti Human HLA DR

Mouse anti Human HLA DR:RPE

Product Type
Monoclonal Antibody
Clone
HL-39
Isotype
IgG3

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Product CodeApplicationsPack SizeList PriceQuantity
MCA1879C F
SDS
0.2 mg
MCA1879PEF
SDS
100 Tests
Mouse anti Human HLA DR antibody, clone HL-39 recognizes HLA DR which is an MHC class II cell surface receptor expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages and activated T lymphocytes.
Mouse anti Human HLA DR antibody, clone HL-39 recognizes a determinant that is dependant upon the association of both the alpha and beta chains of HLA DR.
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class II proteins encoded by the HLA which are HLA DP, HLA DQ and HLA DR.
Bio-rad digital droplet
Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by hydrophobic charge induction chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
Bio-rad
Raji Burkitt lymphoma cell line.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Regulatory
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry1/501/200
Immunohistology - Frozen
Flow CytometryNeat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
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Secondary Antibodies Available

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Useful Reagents Available

DescriptionProduct CodeApplicationsPack SizeList PriceQuantity
Human SeroblockBUF070AF 50 Test
Human SeroblockBUF070BF 200 Test

Product Specific References

References for HLA DR antibody

  1. Horejsí, V. et al. (1986) Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens.
    Tissue Antigens. 28 (5): 288-97.
  2. Philippidis, P. et al. (2010) Anti-inflammatory haemoglobin scavenging monocytes are induced following coronary artery bypass surgery.
    Eur J Cardiothorac Surg. 37: 1360-6.

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